Targeted imaging of esophageal adenocarcinoma with a near-infrared fluorescent peptide

Abstract Background Targeted optical imaging offers a noninvasive and accurate method for the early detection of gastrointestinal tumors, especially flat appearances. In our previous study, sequence SNFYMPL (SNF) was identified as specific peptide to bind esophageal carcinoma using phage-display technology. This study aimed evaluate tumor-targeting efficacy Cy5.5-conjugated SNF probe in vitro vivo. Methods The SNF-Cy5.5 synthesized then High Performance Liquid Chromatography (HPLC) mass spectrometry (MS). Confocal fluorescence Flow cytometry analysis were performed binding specificity receptor affinity OE33. vivo targeting ability carcinoma. Results confocal flow showed that bound specifically plasma membrane OE33 cells with high affinity. vivo, non-block group, exhibited rapid tumor during 24 h p.i. excellent tumor-to-background contrast at 2 For block not observed mice after 4 Ex also revealed higher fluorescent signal intensity value tumors clearly group than (2.6 ± 0.32 × 10 9 vs. 0.8 0.08 , p < 0.05). Conclusions characterized efficiency purity. affinity, specificity, confirmed by tests. is promising adenocarcinoma.